Protocols
Molecular biology
preparation of genomic DNA: quick protocol for single flies (very
fast, good to amplify fragments smaller than 1kb; this genomic DNA can
only be kept for a few months at –20C. Use another protocol if you want
to amplify larger fragments or keep the DNA longer)
using QIAGEN DNeasy Tissue Kit (very good to amplify fragments that are 2-10kb long)
"blue book" protocol (good to amplify 2kb-PCR fragments, the result is a mix of genomic DNA+RNA (no RNAse step))
BDGP protocol
preparation of cDNA from testis: my protocol (did not work well)
Inverse PCR: BDGP protocol
PCR: using ABgene PCR mix
using Roche Expand High Fidelity
Yeast protocols
Staining protocols
muscle dye staining protocols
Antibody staining
antibody staining of embryos: my protocol
antibody staining of embryos with amplification: my protocol (PDF file)
antibody staining of larval gonads and other larval tissues: my protocol
antibody staining of pupal abdominal epidermis: N. Gompel's protocol (PDF file)
antibody staining of pupal abdominal muscles: my protocol
vizualisation of adult abdominal muscles (polarized light, antibody staining, etc.): my protocol
antibody staining of adult ovaries: Horacio's protocol
Emilie's protocol
list of antibodies
In situ staining
Check also http://www-biology.ucsd.edu/~davek/index.html for details about in situ hybridization
preparation of new in situ plasmids and probes: my protocol from RIKEN
fluorescent in situ+antibody staining of embryos: my new protocol my post-doc protocol my PhD protocol
non fluorescent in situ staining of embryos: my protocol
protocols from Leslie Vosshall
Fly organ measurements
my protocols for pupal cases, wings, palps, legs, dry mass
counting ovariole number
Fly genetics
Crosses and genetic markers for my D. simulans-D. sechellia QTL crosses
Behavioral and other assays
resistance to octanoic acid
sperm motility assay (PDF file with picture)
Manuals
RNAlater
plasmid maps