sulfur symbol
Sulphur is more mature than any of the other Principles, and Mercury is not coagulated but by Sulphur: therefore our whole operation in this Art is nothing else but to know how to draw forth that Sulphur out of Metalls, by which our Argent vive in the bowels of the Earth is congealed into Gold, and Silver: which Sulphur indeed is in this work in stead of the Male, but the Mercury in stead of the Female.

A new light of Alchymie: taken out of the fountain of Nature. To which is added a Treatise of Sulphur
Micheel Sandivogius


Metabolic studies
European collaborations

The methionine salvage pathway

Fruit ripening is triggered by production of the gas ethylene. While studying the production of ethylene in apple fruit tissue, Baur and Yang noted that, although methionine was in quantities sufficient for the production of ethylene only for a few hours, ethylene was being produced for months, suggesting a pathway recycling methionine. In plants, this methionine salvage pathway has been known as the methylthioadenosine (MTA) or Yang Cycle at a time when not much was know about the chemical steps in the pathway. Since no volatile sulfur was detected, it was postulated that the atom was recycled to replenish the methionine pool. It was later shown that the methylthio- group of MTA was recycled to methionine via 5'-methylthioribose (MTR) [ Murr 75 , Adams 77 ]. It was initially thought that the methylthio- group was transferred to a four carbon acceptor molecule such as homoserine to regenerate methionine.

Later studies however showed that in plants, like in yeast cells, rat liver and bacterial extracts, the ribose moiety of MTA was also incorporated into the 2-aminobutyrate moiety of methionine.

Yokota and his colleagues, in Nara, Japan, unraveled the chemical intermediates in the pathway present in Bacillus subtilis. When in Hong Kong we developed the genetic analysis of the pathway in B. subtilis, and later in Pseudomonas aeruginosa. In the final touch on the metabolism we established that the salvage cycle is irreversible, via formation and hydrolysis of 2-ketoglutaramate. We have recently written an update of the knowledge of the MSP at the end of 2018.

msp

Methionine (Met) belongs to the twenty amino acids making proteins. It is also required for a great many cellular functions, including initiation of protein synthesis, methylation of DNA, rRNA, a variety of secondary metabolites and xenobiotics, and biosynthesis of cysteine (via the reverse transsulfuration pathway), phospholipids and polyamines. Polyamines, molecules with and aliphatic core and multiple amino groups, are synthesized in large amounts in cells, such as parasites, bacteria and cancer cells.

During polyamine synthesis of ethylene, polyamines and siderophores, methionine is consumed through the utilization of S-adenosylmethionine (AdoMet) which releases 5'-methylthioadenosine (MTA). Since the amount of methionine is typically limiting in cells and de novo synthesis of methionine is energetically expensive, it is important to be able to recycle this amino acid. Methionine regeneration from MTA plays an important role in sustaining the continued production of ethylene and polyamines.

In many organisms, the carbon atoms from the amino acid methionine skeleton do not come from the expected source, aspartate. The methionine salvage pathway is a ubiquitous biochemical pathway (it is present in humans) that maintains methionine levels in vivo by recycling the thiomethyl- moiety of methionine through a degradation pathway that leads from AdoMet through methylthioadenosine (MTA). The ribose ring of MTA provides the carbon skeleton for acyclic intermediates in the salvage pathway. Both of these enzymes were discovered in the laboratory of Abeles. The biochemistry of important enzymes of the pathway is developed in the laboratory of Yokota and his successors.

The methionine salvage pathway is universally used to regenerate methionine from 5’-methylthioadenosine (MTA), a by-product of some reactions involving S-adenosylmethionine. .

mtnN: A Sekowska, A Danchin
Identification of yrrU as the methylthioadenosine nucleosidase gene in Bacillus subtilis
DNA Res (1999) 6: 255-264 PubMed DNA-research

HKU_Pasteur
mtnK: A Sekowska, L Mulard, S Krogh, JK Tse, A Danchin
MtnK, methylthioribose kinase, is a starvation-induced protein in Bacillus subtilis
BMC Microbiol (2001) 1: 15  BMC
HKU_Pasteur
mtnW, mtnX, mtnD, mtnE: A Sekowska, A Danchin
The methionine salvage pathway in Bacillus subtilis
BMC Microbiol (2002) 2:BMC HA F1000

uk-flagMany metabolites are orphan metabolites, in that their becoming has almost never been studied in details. This is the case of methyl-thioadenosine, produced during polyamines synthesis (and production of ethylene in plants). This work in B. subtilis is the first one to use a genetic systematic approach to identify the genes involved in thiomethyl- salvage, in an economical way, without oxidation. It shows that a gene with considerable resemblance to ribulose bisphophate carboxylase oxygenase (RuBisCO, the most abundant enzyme on Earth) is involved in the cycle, and it identifies all the genes that are necessary for the "methionine salvage pathway"

 

fr-flagBeaucoup de métabolites sont "orphelins" en ce que leur devenir n'a pratiquement pas été étudié. C'est le cas du méthyl-thioadénosine, produit au cours de la synthèse des polyamines (et de l'éthylène chez les plantes). Ce travail chez B. subtilis est le premier a utiliser une approche génétique pour tenter d'identifier les gènes impliqués dans le cycle qui permet la récupération du groupement thiométhyl d'une façon économique, sans oxydation. Il montre en particulier qu'un gène très semblable à la ribulose bisphophate carboxylase oxygénase (RuBisCO, l'enzyme la plus abondante de la planète) est impliquée dans le cycle, et il identifie tous les gènes nécessaire au recyclage

mtnW: H Ashida, A Danchin, A Yokota
Was photosynthetic RuBisCO recruited by acquisitive evolution from RuBisCO-like proteins involved in sulfur metabolism?
Res Microbiol (2005) 156: 611-618  pdf

HKU_Pasteur
mtnA, mtnB, mtnC, mtnP: A Sekowska, V Dénervaud, H Ashida, K Michoud, D Haas, A Yokota, A Danchin
Bacterial variations on the methionine salvage pathway
BMC Microbiol (2004) 4:BMC biosapiens

mtnW: H Ashida, Y Saito, T Nakano, N Tandeau de Marsac, A Sekowska, A Danchin, A Yokota
RuBisCO-like proteins as the enolase enzyme in the methionine salvage pathway: functional and evolutionary relationships between RuBisCO-like proteins and photosynthetic RuBisCO
J Exp Bot (2008) 59: 1543-1554
PubMed

HKU_Pasteur
mtnW: Y Saito, H Ashida, T Sakiyama, N Tandeau de Marsac, A Danchin, A Sekowska, A Yokota
Structural and functional similarities between a RuBisCO-like protein from Bacillus subtilis and photosynthetic RuBisCO
J Biol Chem (2009) 284:1 3256-13264 PubMed pAp

Finally, because methionine is costly to make, it is important to prevent its degradation. This result is achieved by the final step of the salvage pathway, where aminotransferase MtnV uses glutamine instead of glutamate as the alpha-amino group donor, producing alpha-ketoglutaramate. In turn, this metabolite is hydrolyzed into ammonium and alpha-glutarate that enters the TCA cycle, by omega-amidase (EC 3.5.1.3) MtnU. Because water is present at a 55 M concentration this step is, in practice, irreversible, preventing formation of the alpha-ketoacid precursor of methionine.

mtnU: E Belda E, A Sekowska, F Le Fèvre, A Morgat, D Mornico, C Ouzounis, D Vallenet, C Médigue, A Danchin
An updated metabolic view of the Bacillus subtilis 168 genome
Microbiology (2013) 159: 757-770. doi: 10.1099/mic.0.064691-0 pubmed

 A Danchin, A Sekowska
The logic of metabolism and its fuzzy consequences
Environ Microbiol (2014) 16: 19-28 pubmed

A Sekowska, H Ashida, A Danchin
Revisiting the methionine salvage pathway and its paralogues
Microb Biotechnol. (2018) 12: 77-97 doi: 10.1111/1751-7915.13324


       accueil