PNS expression patterns (3)

Gal4 lines

    Expression pattern in the embryonic PNS at stage 15-17 is schematized here. Black cells are cells that accumulate high levels of the marker, whereas grey cells accumulate low levels and white cells do not accumulate the marker. Cells for which the expression has not been studied are represented by a light grey line. Expression pattern at earlier embryonic stages is also mentioned in the text below each diagram.
     Click on a marker name to link to the corresponding FlyBase page.



C161-GAL4

c929-gal4











Expression is not detected in embryonic stages (Shepherd and Smith, 1996). In the larva, C161-GAL4  is strongly expressed in five dorsal multidendritic neurons (Shepherd and Smith, 1996): ddaA, ddaB, ddaD, ddaE and ddaF (Williams and Truman, 20005). In the ventral and lateral regions, C161-GAL4 is expressed in vdaA, vdaB, vdaC, vdaD, vbd, vpda, lbd, ltd, ldaA, ldaB and in a scolopale of a lateral chordotonal organ (Shepherd and Smith, 1996). This P-element is inserted into the smallminded gene (Long et al., 1998).

Expression is detected in the lbd neuron (Hewes et al., 2003).  This P element is inserted in the dimmed gene (Hewes et al., 2003).





Gal4221
Gal4477
Gal4109(2)80











It accumulates at a high level in the class I md neurons (vpda, ddaD and ddaE) and at a low level in the class IV md neurons (ddaC, v'ada and vdaB) (Grueber et al., 2003; W. Grueber, personal observations). Its accumulation pattern in the vtd neurons has not been investigated.
It accumulates in the class IV md neurons (vmd1a, vmd4a and ddaC) (Grueber et al., 2003). Same pattern as Collier, B6-2-25 and Pickpocket Its accumulation pattern in the vtd neurons has not been investigated.
It accumulates in all the md neurons (Gao et al., 1999) but not in es neurons (Brenman et al., 2001). This Gal4 line starts driving transgene expression at stage 15 (Sugimura et al., 2004).





IG1-1

IG1-2











It accumulates in the ddaE and vpda neurons (a subset of class I neurons) (Sugimura et al., 2003). This Gal4 line starts driving transgene expression at stage 15 (Sugimura et al., 2004).
It accumulates in all the md neurons but not in ch or es neurons (Sugimura et al., 2003). This Gal4 line starts driving transgene expression at stage 15 (Sugimura et al., 2004).






NP1161
NP2225
NP7028











It accumulates in the class IV md neurons (vmd1a, vmd4a and ddaC) (Sugimura et al., 2004). This Gal4 line starts driving transgene expression at stage 15 (Sugimura et al., 2004).
It accumulates in the class I md neurons (ddaD, ddaE and vpda) (Sugimura et al., 2003). This P-element is inserted into the abrupt gene (Sugimura et al., 2004). This Gal4 line starts driving transgene expression at stage 15 (Sugimura et al., 2004).
It accumulates in the class IV md neurons (vmd1a, vmd4a and ddaC) (Sugimura et al., 2003). This Gal4 line starts driving transgene expression at stage 15 (Sugimura et al., 2004).





ppk1.9GAL4











The ppk1.9GAL4 transgene was constructed using a 1.9-kb DNA fragment located just upstream of the predicted ppk translation start site. ppk1.9GAL4 is expressed in the class IV md neurons vmd1a, vmd4a and ddaC (Ainsley et al., 2003).











References

Ainsley JA, Pettus JM, Bosenko D, Gerstein CE, Zinkevich N, Anderson MG, Adams CM, Welsh MJ, Johnson WA. 2003. Enhanced locomotion caused by loss of the Drosophila DEG/ENaC protein Pickpocket1. Curr Biol. 13, pp. 1557-63.  Medline

Brenman JE, Gao FB, Jan LY, Jan YN. 2001. Sequoia, a tramtrack-related zinc finger protein, functions as a pan-neural regulator for dendrite and axon morphogenesis in Drosophila. Dev Cell. 1(5):667-77. Medline

Gao FB, Brenman JE, Jan LY, Jan YN. 1999. Genes regulating dendritic outgrowth, branching, and routing in Drosophila. Genes Dev. 13, pp. 2549-61. Medline

Grueber, W. B., Jan, L. Y. and Jan, Y. N. 2003. Different levels of the homeodomain protein cut regulate distinct dendrite branching patterns of Drosophila multidendritic neurons. Cell 112, pp. 805-18. Medline

Hewes RS, Park D, Gauthier SA, Schaefer AM, Taghert PH. 2003. The bHLH protein Dimmed controls neuroendocrine cell differentiation in Drosophila. Development 130(9):1771-81. Medline

Long, A.R., Yang, M., Kaiser, K., Shepherd, D. 1998. Isolation and characterisation of smallminded, a Drosophila gene encoding a new member of the Cdc48p/VCP subfamily of AAA proteins. Gene 208, 191–199. Medline

Shepherd D, Smith SA. 1996. Central projections of persistent larval sensory neurons prefigure adult sensory pathways in the CNS of Drosophila. Development 122, pp. 2375-84.  Medline

Sugimura, K., Yamamoto, M., Niwa, R., Satoh, D., Goto, S., Taniguchi, M., Hayashi, S. and Uemura T. 2003. Distinct developmental modes and lesion-induced reactions of dendrites of two classes of Drosophila sensory neurons. J Neurosci. 23, pp. 3752-60. Medline

Sugimura, K., Satoh, D., Estes, P., Crews, S. and Uemura, T. 2004. Development of Morphological Diversity of Dendrites in Drosophila by the BTB-Zinc Finger Protein Abrupt. Neuron 43, pp. 809-22. Medline

Williams DW, Truman JW. Cellular mechanisms of dendrite pruning in Drosophila: insights from in vivo time-lapse of remodeling dendritic arborizing sensory neurons.2005. Development 132, pp. 3631-42. Medline














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