Quick genomic DNA prep for wings of single flies
used in McGregor et al., 2007
(based on Gloor et al., 1997 and Gleason et al., 2004)

great protocol if you want to genotype flies and keep them alive, it is like taking a piece of the tail for mouse geneticists...
This genomic DNA can only be kept for a few months at –20C.

squishing buffer (100mL):       1mL Tris Hcl 1M pH=8.2
                                                200uL EDTA 0.5M
                                                500uL NaCl 5M
                                                94mL milliQ water
               autoclave.


1. prepare the solution: 100uL squishing buffer+1uL proteinaseK (20mg/mL) +5uL RNAse (20mg/mL). 5ul is needed for each fly.
2. anesthetize flies to be genotyped and cut the wings off using fine-point tweezers. Remove as much of the wings as possible without killing the fly. Put the two wings into a small PCR tube. Store at -20C.
3. take 5uL of the squishing buffer+proteinase K+RNAse with a pipette.
4. grind the wings (as much as you can see) with the pipette tip and then expell the 5uL into the tube.
5. incubate the squished wings at 37C for 25min.
6. inactivate proteinase K at 95C for 1-2min.
7. store at -20C. Use 1uL for a 25uL-PCR reaction.