| Protocols
of V. Courtier-Orgogozo Lab Molecular biology preparation of genomic DNA: quick protocolfor singleflies (very fast, good to amplify fragments smaller than 1kb; this genomic DNA canonly be kept for a few months at –20C. Use another protocol if you want to amplify larger fragments or keep the DNA longer) quick protocolfor flywings (great if you want to keep the flies alive!!!) using QIAGEN DNeasy Tissue Kit (very good to amplify fragments that are 2-10kb long) "blue book"protocol (good to amplify 2kb-PCR fragments, the result is a mix of genomic DNA+RNA (no RNAse step)) BDGP protocol protocol usingDNAzol DNAzol sheet 1 DNAzol sheet 2 extraction of RNA: using Promega kit preparation ofcDNA: using Ambion kit (did not work well), other protocolusing Promega kit (OK) Inverse PCR: BDGP protocol PCR: using ABgene PCR mix using Roche Expand High Fidelity using degenerate primers (M. Koelle advices, David Towles protocol, HCGS protocol) PCR primer design general protocols: A. Moore lab C. D-V lab Staining protocols muscle dye staining protocols Antibody staining antibody staining of embryos: my protocol antibody staining of embryos with amplification: my protocol (PDF file) antibody staining of larval gonads and other larval tissues: my protocol antibody staining of pupal abdominal epidermis: N. Gompel's protocol (PDF file) antibody staining of pupal abdominal muscles: my protocol vizualisation of adult abdominal muscles (polarized light, antibodystaining, etc.): my protocol antibody staining of adult ovaries: Horacio's protocol Emilie's protocol list of antibodies In situ staining Check also http://www-biology.ucsd.edu/~davek/index.html fordetails aboutin situ hybridization preparation of new in situ plasmids and probes: my protocol from RIKEN my current protocol fluorescent in situ+antibody staining of embryos: my newprotocol my post-doc protocol my PhDprotocol non fluorescent in situ staining of embryos: old protocol protocol inC. D-V. lab protocols from Leslie Vosshall Fly food Banana-opuntia flyfood recipe Fly organ measurements my protocols for pupal cases, wings, palps, legs, dry mass counting ovariolenumber Fly genetics Crosses and genetic markers for my D. simulans-D. sechellia QTL crosses Yeast protocols yeast media recipes html PDF geneticin my protocols primers erg2mutants yeast deletion strains Behavioral and other assays resistance to octanoic acid sperm motilityassay (PDF file with picture) Manuals RNAlater pGEM-T (PDF) low massladder plasmid maps: pBluescript IIKS- pBluescript IISK- pBluescript IISK+ pcaSpeR-hs |